For isolation of CD62L+ T cells from peripheral blood mononuclear cells. The kit includes all Fab Streptamer® components for positive cell isolation with magnetic microbeads*and for removal of Streptamers from isolated cells.

1.) Strep-Tactin® Magnetic Microbeads for Fab Streptamers; 2 vials for 5x10^8 cells each

2.) CD62L Fab-Strep, human for isolation of T-cells; 2 vials for 5x10^8 cells each

3.) D-Biotin stock solution for Streptamer® technology; 2x 1 ml

4.) Buffer IS, Streptamer® Washing Buffer, 10x concentrated; 50 ml

This product requires dry ice shipment and is shipped directly from our Headquarters in Germany.

*Microbeads are intended for use with a permanent magnet for manual cell isolation; we recommend our StrepMan magnet for 2 x 15 ml and 2 x 50 ml Falcon tubes.

Principle of Selection:

Isolation of human CD62L+ T cells is performed by positive selection. PBMCs are labeled with magnetic CD62L Fab Streptamers. Labeled cells are isolated in a strong magnet (StrepMan, horizontal position) where they migrate toward the tube wall on the side of the magnet. This CD62L positive cell fraction is collected and cells are liberated from all labeling reagents by addition of biotin in a strong magnet (StrepMan, upright position).The magnetic Streptamers migrate toward the tube wall and the label-free cells remain in the supernatant. Biotin is removed by washing.

The resulting cell preparation is highly enriched with CD62L+T cells with a purity of more than 90%. No depletion steps and no columns are needed.

IBA-lifesciences

二十多年来IBA 为生命科学应用提供创新技术。该产品组合包括用于细胞选择和扩展、蛋白质生产和分析的工具，以及定制的寡糖和预定义的DNA/RNA。

For more than two decades IBA provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.