The kit allows the selection of CD19+ B cells directly from whole blood, buffy coat or PBMCs.

The Kit includes:

• 4x Fab-TACS® Gravity column (Capacity: 1x10^8 target cells per column)
• CD19 Fab-Strep, human
• Buffer CI (10x)
• Biotin stock solution
• 1x Fab-TACS® Gravity Adapter

The Fab-TACS® Gravity column is filled with a Strep-Tactin® coated matrix made of cell-grade agarose. Strep-tagged Fab fragments (Fab-Streps) specifically bind to the matrix. Subsequently, samples pass through the column. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to the target cell. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces. The label-free authentic target cells are now ready for further downstream applications.

IBA-lifesciences

二十多年来IBA 为生命科学应用提供创新技术。该产品组合包括用于细胞选择和扩展、蛋白质生产和分析的工具，以及定制的寡糖和预定义的DNA/RNA。

For more than two decades IBA provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.