Strep-Tactin® Magnetic Beads are polysaccharide coated magnetic microspheres covalently linked to Strep-Tactin®. The paramagnetic beads enable a rapid and economic cell isolation at 4 °C under sterile conditions.
The microbeads are designed for cell isolation with a permanent magnet (e.g. IBA s StrepMan magnet). For receptor-specific isolation of eukaryotic cells, Strep-Tactin® Magnetic Beads have to be combined with Fab-Streps, Fab fragments containing the Strep-tag® affinity tag. After isolation of the cells Strep-Tactin® Magnetic Beads can be mildly dissociated from the Fab-Streps using biotin. The monomeric Fab-Streps then spontaneously dissociate from the cell receptor due to its very weak binding. The rapid and complete dissociation of all Streptamer® reagents from the cells assures full functionality of the isolated cells. Due to the reversibility, the cells can be sequentially positively isolated with different Fab-Streps.
For isolation of antigen-specific CD8 T-cells, Strep-Tactin® Magnetic Beads have to be combined with MHC I-Streps, antigen-loaded MHC I containing the Strep-tag®. After isolation of T-cells, Strep-Tactin® Magnetic Beads can be mildly dissociated from the MHC I-Streps using biotin. The monomeric MHC I-Streps then spontaneously dissociate from the T-cell receptor due to its very weak binding. The rapid and complete dissociation of all Streptamer® reagents from the T-cells assures full functionality of the isolated cells. Due to the reversibility, the cells can be sequentially positively isolated with different MHC I-Streps.
This product requires wet ice shipment and is shipped directly from our Headquarters in Germany.
IBA-lifesciences
二十多年来IBA 为生命科学应用提供创新技术。该产品组合包括用于细胞选择和扩展、蛋白质生产和分析的工具,以及定制的寡糖和预定义的DNA/RNA。
For more than two decades IBA provides innovative technologies for life science applications. The product portfolio includes tools for cell selection & expansion, protein production & assays as well as custom oligos & predefined DNA/RNA.
品牌 | 货号 | 名称 | 规格 |
IBA-lifesciences | 6-5510-050 | Strep-Tactin® Magnetic Microbeads | for isolation out of 5x10^8 cells |
IBA-lifesciences | 6-8000-201 | CD3 Fab Streptamer® Isolation Kit MB, human | for isolation out of 1x10^9 cells with magnetic microbeads (MB) |
IBA-lifesciences | 6-8000-203 | CD8 Fab Streptamer® Isolation Kit MB, human | for isolation out of 1x10^9 cells with magnetic microbeads (MB) |